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. 2018 Nov 26;9(12):1161. doi: 10.1038/s41419-018-1203-8

Fig. 1. Microcarrier bead -culture of OEhMSCs and MOSJ cells in a RWV:

Fig. 1

a Cells attached to collagen I-coated beads before co-culture in the RWV. Micrographs i–iv illustrate beads loaded with OEhMSCs, micrographs v–viii illustrate beads loaded with MOSJ-Dkk1 cells. Phase (i, v), GFP (ii), calcein AM staining for non-GFP-labeled OEhMSC live cells (iii), and staining of dead cell nuclei (iv). Phase (v), RFP (vi), calcein AM staining for live MOSJ cells (vii) and Hoescht staining of dead MOSJ nuclei (iv). b–d Co-cultures of OEhMSCs and MOSJ-Dkk1 cells on collagen I-coated beads. Low- (b), mid- (c), and high-power (d) images indicating presence of RFP-labeled MOSJ-Dkk1 (right) and GFP-labeled OEhMSCs (center), with both merged with phase images (left). High-power micrograph in d, indicates (arrowed) OEhMSC-laden bead with an OEhMSC forming a bridge with a MOSJ-Dkk1 laden bead