Fig. 2. Osteogenic activity of OEhMSCs cultured in the presence and absence of MOSJ-Dkk1 or MOSJ-pLenti cells on collagen I.

a, c, e, g RWV co-cultures. b, d, f, h Monolayer co-cultures. a, b Enumeration of OEhMSCs (refers to 50 cm² growth area). c, d Enumeration of MOSJ cells (refers to 50 cm² growth area). e, f Secretion of OPG as measured by ELISA. g, h ALP activity by OEhMSCs cultured for up to 8 days in the presence or absence of MOSJ cells. The label “OEhMSC” refers to OEhMSC monoculture and “+ MOSJ…” refers to co-culture of MOSJ and OEhMSC cells. Statistics: data presented with means and standard deviations. Comparisons were ANOVA with Tukey’s post test. P < 0.05*, <0.01**, <0.005***