Skip to main content
. 2018 Nov 26;7(11):93. doi: 10.1038/s41389-018-0104-0

Fig. 5. Low glutamine induces phosphorylation of p53 on Ser392.

Fig. 5

a HT1080 cells cultured in complete medium (Comp), glutamine-free (-Q) medium or complete medium with 2 μM Doxorubicin (Doxo) overnight. Cell lysate was collected for immunoblotting using the indicated antibodies. b Time course of p53 phosphorylation on Ser392 using HT1080 cells cultured in glutamine-free medium for 0, 1, 3, 6, 8, and 18 h. Cell lysate was collected for immunoblotting using the indicated antibodies. c Time course of p53 activation on Ser389 using wild-type and Ikkβ−/− MEFs cultured in glutamine (Gln)-free medium for 0, 1, 3, 6, 8, and 18 h. Cell lysate was collected for immunoblotting using indicated antibodies. d HT1080 cells transduced with shRNA against control (Ctrl) or IKKβ were cultured in complete medium, glutamine-free medium, complete medium with 2 μM Doxo, or glutamine-free medium with 200 nM TPCA-1 (-Q + TPCA) for 6 h, or complete medium with 15 ng/ml TNFα for 15 min. Cell lysate was collected for immunoblotting with indicated antibodies