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. 2018 Nov 26;8:17347. doi: 10.1038/s41598-018-35485-2

Figure 5.

Figure 5

IL-34 enhanced oxLDL-induced inflammation in BMDMs. (a) BMDMs were pre-incubated with IL-34 (50 ng/ml) or oxLDL (50 μg/ml) for the indicated times. IL-6, TNF-α and IL-1β mRNA expression were quantified by RT-PCR. (b) BMDMs were pre-incubated with IL-34 (50 ng/ml) or oxLDL (50 μg/ml) for the indicated times and then the cells were activated by lipopolysaccharide (LPS) for an additional 3 hr. Supernatants were collected after stimulation and IL-6, TNF-α and IL-1β concentrations were measured by ELISA. Data represent mean ± SD of n = 3 biologically independent experiments. *P < 0.05, **P < 0.01.