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. 2018 Nov 26;8:17345. doi: 10.1038/s41598-018-35559-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MTT assay. Effect of DOX alone (1, 2, 4, 10, and 20 μM), PTX alone (1, 2, 4, 10, and 20 μM), and α-Lac (1, 2, 4, 10, and 20 μM) along with DOX or PTX (1, 2, 4, 10, and 20 μM) with equal (1:1, 2:2, 4:4, 10:10, and 20:20) and ascending ratios (1:2, 1:4, 1:10, and 1:20) in MDA-MB-231 (a–f) and T47D (g–l) cells. The cells were incubated with the described concentrations and ratios of α-Lac, DOX, and PTX for 24 (a,b,g and h), 48 (c,d,i and j), and 72 (e,f,k and l) (h). Cell viability was evaluated by MTT assay. The percent viability data were normalized to the corresponding control. The data represents an average ± SEM of three independent experiments. *represents p < 0.05 compared to the corresponding control.