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. 2018 Nov 26;8:17357. doi: 10.1038/s41598-018-35646-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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High-speed camera imaging of the movement of HeLa cells through the constricted portion of the microchannel. (a) Motion of a single cell: (i) a single drop approaching the constriction, (ii) cell entering the constriction, (iii) and (iv) cell moving inside the constriction, (v) cell squeezing out of the constriction and (vi) cell flowing outside the constriction. (b) Motion of three cells: (i) three cells approaching the constriction, (ii) and (iii) cells entering the constriction one by one, (iv) and (v) cells travelling inside the constriction and moving out one by one and (vi) cells flowing outside the constriction. (c) Motion of aggregated cells: (i) aggregated cells approaching the constriction, (ii) and (iii) cells entering the constriction in a queue and travelling through it and (iv–viii) cells entering through the constriction and moving out one by one to re-form the aggregated cluster of HeLa cells.