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. 2018 Nov 26;9:4991. doi: 10.1038/s41467-018-07529-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Genetic differentiation of PTPN1. a Principle component analysis plot of geographically different locust populations based on the SNPs of the PTPN1 gene region. Lowland-S and Lowland-N represent South and North China lowland locust populations, respectively. b Multi-species alignment of amino acid sequence of PTP1B encoded by PTPN1. Oedaleus asiaticus and Schistocerca gregaria are two outgroup species closely related to Locusta migratoria (abbreviated as Lm). The missense mutation p.Asn349Ile (N349I) is located at the proline-rich (Pro-Rich) domain of PTP1B. c Mutation frequency of p.Asn349Ile in Tibetan and lowland locusts. The pie chart shows the proportion of p.Asn349Ile allele frequency in each population. Sample size: n ≥ 38. d Allele frequency of mutant loci in PTP1B. I/I, I/N, and N/N represent homozygous wild type, heterozygote mutant, and homozygous mutant for p.Asn349Ile, respectively. Maps were generated using DIVA-GIS (http://www.diva-gis.org/)