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. 2018 Nov 26;8:17379. doi: 10.1038/s41598-018-35690-z

Figure 5.

Figure 5

Cryosections of mice superior cervical ganglia quadruple-labelled to show chemical coding of postganglionic neurons and FFAR3-expressing neurons. Quadruple-staining of SCG sections were performed with Anti-Tyrosine hydroxylase (Anti-TH, a) Anti-FFAR3 (b) Anti-Neuropeptide Y (Anti-NPY, c) and Anti-Substance P (Anti-SP, d). White arrow indicates a representative neuron SP-, TH-, NPY- and FFAR3- IR and presumed to be a vasoconstrictor neuron. Labeling was repeated twice and using four wild-type animals on each replicate. Scale bars in all images are 20 μm.