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. 2018 Nov 20;12:437. doi: 10.3389/fncel.2018.00437

Figure 6.

Figure 6

Activation of AKT by IGF-1 reverses the inhibitory effect of CT04 on SC proliferation. The SCs were treated with CT04 in the presence or absence of IGF-1 for 24 h. (A,B) Western blotting was performed to confirm the effect of IGF-1 on activation of AKT. GAPDH was used as the loading control (n = 4, *P < 0.05). (C-L) Analysis of EdU incorporation indicated that IGF-1 partly restored the declined EdU positive ratio in CT04 treated cells (n = 15, *P < 0.05). (M) Statistics of cell density displayed that CT04-mediated inhibition of SC density was reversed by IGF-1 (n = 15, *P < 0.05). (N) WST-1 assay revealed that IGF-1 treatment partly restored the change in absorbance value caused by CT04 (n = 4, *P < 0.05). (O) Western blots showed that application of IGF-1 up-regulated the expression of PCNA in the CT04 treated cells. The blots were cropped from different parts of the same gel. (P) Statistical diagram of relative protein expression of PCNA (n = 4, *P < 0.05). The expression levels of target proteins in the control group were normalized to 1.