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. 2018 Nov 20;12:437. doi: 10.3389/fncel.2018.00437

Figure 7.

Figure 7

Activation of AKT by SC79 counteracts the inhibitory effect of CT04 on SC proliferation. SCs were treated with DMSO as vehicle control. The SCs were treated with CT04 with or without SC79 for 24 h. (A,B) Western blotting was performed to confirm the effect of SC79 on activation of AKT (n = 4, *P < 0.05). (C-L) The EdU incorporation assay suggested that application of SC79 increased the EdU positive ratio in CT04 treated cells (n = 15, *P < 0.05). (M) The statistics showed that CT04-mediated suppression of SC density was partly restored by SC79 (n = 15, *P < 0.05). (N) Assessment of cell proliferation by WST-1 assay displayed that SC79 counteracted the inhibitory effect of CT04 on SC proliferation (n = 4, *P < 0.05). (O,P) Western blots and statistical data indicated that the addition of SC79 increased the expression of PCNA in the CT04 treated cells (n = 4, *P < 0.05). The blots were cropped from different parts of the same gel. The expression levels of target proteins in the control group were normalized to 1.