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. 2018 Nov 26;8:17373. doi: 10.1038/s41598-018-35775-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative quantification of the O. tsutsugamushi 56 kDa gene in PBMCs and eschars. The expression level of the 56 kDa gene was evaluated by nested qRT-PCR. The data were normalized to the Gapdh dCT value of the non-infected sample of normal subject #1. The error bars represent the SD of three technical replicates of each sample.