. 2018 Nov 26;8:17397. doi: 10.1038/s41598-018-35688-7

Table 1.

Kinetic parameters of purified wild type CV_TA (WT) and CV_Y168F (Y168F) towards the amino group donor 1-PEA and amino acceptor 2-OOA.

Substrate	K_cat^c (min⁻¹)		K_m (mM)		K_cat/K_m (min⁻¹mM⁻¹)
Substrate	WT	Y168F	WT	Y168F	WT	Y168F
1-PEA^a	28.6 ± 0.3	29.2 ± 5.4	2.6 ± 0.63	1.1 ± 0.3	11.1	25.7
2-OOA^b	24.7 ± 4.4	21.5 ± 1.7	0.4 ± 0.15	0.2 ± 0.07*	61.8	126.5

^aReactions with different 1-PEA concentration: 0.1, 0.25, 0.5, 0.75, 1, 2, 2.5, 4, 10, 15 and 20 mM at 45 °C and pH 7.

^bReactions with different amino acceptor concentration: 0.1, 0.25, 0.5, 0.75, 1, 2, 2.5, 4, 10, 15 and 20 mM at 45 °C and pH 7.

^cK_cat is expressed per active site of CV_TA as it exists as a homodimer with two active sites.

All values are a mean of three independent determinations.

*Statistically significant comparing to the wild type (p < 0.05) by using t-test.