. 2018 Nov 26;8:17397. doi: 10.1038/s41598-018-35688-7

Table 3.

Kinetic parameters of purified CV_W60C/Y168F.

W60C/Y168F	K_cat* (min⁻¹)	K_m (mM)	K_cat/K_m (min⁻¹mM⁻¹)
1-PEA^a	3.4 ± 0.3	4.4 ± 0.1	0.8
2-OOA^b	10.9 ± 0.7	0.04	272.5

^aHalf-reaction monitored with different 1-PEA concentration: 0.1, 0.25, 0.5, 0.75, 1, 2, 2.5, 4, 10, 15 and 20 mM, with 1 mg/ml TA at 45 °C and pH 7.

^bReactions with different 2-OOA concentrations: 0.1, 0.25, 0.5, 0.75, 1, 2, 2.5, 4, 10, 15 and 20 mM at 45 °C and pH 7.

*The K_cat is expressed per active site of CV_TA as it exists as a homodimer with two active sites.

All values are a mean of three independent determinations.