Table 5.

Primers used for site directed mutagenesis of CV_TA.

Primer	Sequence (5′-3′)
A231S (F)	CATCCAGGGCTCCGGCGGCGTGATC
A231S (R)	GATCACGCCGCCGGAGCCCTGGATG
A231T (F)	CATCCAGGGCACCGGCGGCGTGATC
A231T (R)	GATCACGCCGCCGGTGCCCTGGATG
R416K (F)	AACAACCTGATCATGAAGGCATGCGGCGACCACATC
R416K (R)	GATGTGGTCGCCGCATGCCTTCATGATCAGGTTGTT
S156A (F)	GGCTATCACGGCGCCACCATCGGCG
S156A (R)	CGCCGATGGTGGCGCCGTGATAGCC
W60C (F)	ATGGCCGGACTGTGCTGCGTGAACGTC
W60C (R)	GACGTTCACGCAGCACAGTCCGGCCAT
Y168F (F)	GGCGGCATGAAGTTCATGCACGAGCAG
Y168F (R)	CTGCTCGTGCATGAACTTCATGCCGCC