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. 2018 Nov 26;8:17375. doi: 10.1038/s41598-018-35745-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hypoxia exposure up-regulates the expression of sFlt-1 variants in human trophoblast-derived choriocarcinoma cell lines. BeWo, JAR, and JEG-3 cells were incubated for 24 h under normoxic or hypoxic conditions. The mRNA expression levels of all Flt-1 transcript variants (FLT-1) and three splice variants of the Flt-1 gene in these cells were determined by quantitative real-time PCR analysis using β-actin mRNA as a reference. (A) FLT-1 mRNA expression level in the three trophoblast-derived choriocarcinoma cell lines. Results are expressed as a fold change relative to the cells under normoxic conditions. (B–D) The mRNA expression levels of three Flt-1 splice variants (tmFlt-1, sFlt-1 i13, and sFlt-1 e15a) in BeWo (B), JAR (C), and JEG-3 (D) cells. Results are represented as a ratio relative to the expression of β-actin mRNA. All values are represented as the means ± SD (n = 3).