Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 26;8:17375. doi: 10.1038/s41598-018-35745-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Inhibition of HIF-2α by siRNA transfection decreases hypoxia-induced up-regulation of Flt-1 gene expression in BeWo and JEG-3 cells. BeWo and JEG-3 cells were transfected with 10 nM of control siRNA, HIF-1α siRNA, or HIF-2α siRNA. Seventy-two hours after transfection, cells were incubated for 24 h under normoxic or hypoxic conditions. (A,B) Evaluation of siRNA-mediated HIF-1α/2α mRNA knockdown in BeWo (A) and JEG-3 (B) cells. HIF-1α/2α mRNA expression levels were measured by quantitative real-time PCR analysis using β-actin mRNA as a reference. Results are expressed as a percentage relative to control siRNA (siCont)-transfected cells under normoxic or hypoxic conditions. (C,D) HIF-1α/2α protein expression was assessed in BeWo (C) and JEG-3 (D) cells by Western blot analysis. β-actin was used as a loading control. Uncropped images of Western blots are presented in Supplementary Fig. S5. (E, F) The mRNA expression of all Flt-1 transcript variants (FLT-1) was measured in BeWo (E) and JEG-3 (F) cells by quantitative real-time PCR analysis. FLT-1 mRNA expression level was calculated by normalizing to β-actin mRNA and represented as a fold change relative to control siRNA (siCont)-transfected cells under normoxic conditions. All values are represented as the means ± SD (n = 3). Asterisks indicate a significant difference (P < 0.05).