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. 2018 Nov 26;8:17366. doi: 10.1038/s41598-018-35673-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The process of measuring the anti-FS immune response on frameshift peptide arrays. Cancer patient sera was incubated overnight on the frameshift peptide array, fluorescence labeled secondary antibody was then used for transforming antibody-FSP binding signal to fluorescence, each slide contains 24 arrays.