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. 2018 Nov 26;8:17366. doi: 10.1038/s41598-018-35673-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reactive FSP induced T cell response and it was related to tumor protection in 4T1 breast cancer model. (A) IFN-γ Positive spots for media only and reactive FSP (R Pep) in 10 mice (M1-M10) of reactive FSP group. P-value < 0.01 with student’s two-tail t-test. (B) Tumor volume was linearly fitted against IFN-γ Positive Spots from ELISPOT experiments, p-value = 0.51. (C) 10 mice were separated into Small Tumor group (n = 4) and Large Tumor group (n = 6), p-value < 0.01 with student’s two-tail t-test. (D) Positive Spots from Small Tumor group were compared to Large Tumor group, p-value < 0.05 with student’s two-tail t-test. All error bars represent Mean ± SEM.