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Overview of storage conditions for each of the three separate donor feces. 45 fecal samples were obtained from each donor feces. The samples were processed in triplicates and either stored directly at −80 °C, at −20 °C for 24 or 72 hours, or in one of the following buffers: DNA/RNA Shield, PSP Buffer, or RNAlater at 4 °C/RT, prior to freezing at −80 °C. Red numbers indicate number of samples that progress to this storage methodology.
