Fig. 4.

Androgen/AR epigenetically attenuates HIF1-mediated GPI transcription in hypoxia. LNCaP-AdtHs cells were treated with basal (CSS media), 1% O2 (hypoxia), 1 nM R1881 (androgen), hypoxia + androgen (Combo), or Combo + androgen/AR-targeted agents (enzalutamide or JQ1) for 48 h. Afterwards, gene expressions (GPI and ENO1) were determined by qRT-PCR and the enrichment of epigenetic compositions at the HRE of GPI and ENO1 promoters was determined by ChIP. a The mRNA level of GPI and ENO1 at the indicated conditions. b Levels of AR enrichment at the HRE regions in GPI and ENO1 promoters. c Levels of 2-methyl histone 3 lysine 9 enrichment at the HRE regions in GPI and ENO1 promoters. d Levels of HIF1α binding to the HRE regions in GPI and ENO1 promoters. e Levels of acetylation of histone 3 lysine 9 enrichment at the HRE regions in GPI and ENO1 promoters. f Levels of p300 enrichment at the HRE regions in GPI and ENO1 promoters. g Levels of RNA polymerase II enrichment at the HRE regions in GPI and ENO1 promoters. All values were mean ± s.d. relative to the basal condition, n = 3, ^#, *P < 0.05, **P < 0.01, NSD, two-sided t-test