. 2018 Nov 20;9:2786. doi: 10.3389/fmicb.2018.02786

Table 1.

Oligonucleotide primers used to generate GBS mutagenesis and complementation vectors.

Primer name	Sequence (5′ to 3′)
npx-P1^a	CCGCGGATCCCCAAAGCCCCAGATTTTGCTG
npx-P2^b	CCCATCCACTAAACTTAACAAAGAGGATTGGGCTATGCGA
npx-P3^b	TGTTTAAGTTTAGTGGATGGGACCATAAAGTCGGTCTCAGCA
npx-P4^c	GGGGGTACCTTAGACCCCATTATGAGGCTGC
npx-P5	CTAAGGCTGCGTCTAACCGT
npx-P6	TGCTGTAGCTATTGCAGCGT
npx-pLZ12-F2^a	CGCGGATCCAGGAGGACAGCTATGACAGAAAAATATGTA
npx-pLZ12-R2^d	AAAACTGCAGCTATTCGCATAGCCCAATCC

^aBamHI restriction site is shown in bold. ^bComplementary sequences for P2 and P3 sets are underlined. ^cKpnI restriction site is shown in bold. ^dPstI restriction site is shown in bold.