Table 3.

DNA sequence of primer pairs used in PCR assays in human, rat and mice tissue.

Gene name	Primer sequence forward (5′−3′)*	Primer sequence reverse (5′−3′)*	Annealing temperature (°C)	Efficiency(%)
Rattus norvegicus pre-mir-146A	GCTCTGAGAACTGAATTCCATGGGT	ATGACGATAGAGCTATCCCAGC	61	72.1
Mus musculus pre-mir146A	GCTCTGAGAACTGAATTCCATGGGT	AGCTGAAGAACTGAATTTCACAGGTC	61	81.7
Homo sapiens, Rattus norvegicus and Mus musculus RNU6-1	GCTCGCTTCGGCAGCACATATACTAA	ACGAATTTGCGTGTGTACTCCTTGCG	69	91.1
Homo sapiens mature MIR146a	UGAGAACUGAAUUCCAUGGGUU	Universal primer (Quiagen Cat. N° 1046471)	55	90.6
Mus misculus mature Mir146a	UGAGAACUGAAUUCCAUGGGUU	Universal primer (Quiagen Cat. N° 1046471)	55	113
Rattus norvegicus mature Mir146a	UGAGAACUGAAUUCCAUGGGUU	Universal primer (Quiagen Cat. N° 1046471	55	90.3
Control snRNA RNU6B	Quiagen Cat. N° MS00033740	Quiagen kit Cat. N° MS00033740	55	103.5
Mus musculus Ifng	GCTCTGAGACAATGAACGCTACAC	TTCTTCCACATCATGCCACTTGAG	59.2	100.7
Mus musculus Ccl2	CAAGATGATCCCAATGAGTAG	TTGGTGACAAAAACTACAGC	60	96.5
Mus musculus S100a8	GCCCTCTACAAGAATGACTTCAAG	ATCACCATCGCAAGGAACTCC	60	91.4
Mus musculus Alpi	CATGGACATTGATGTGATCC	AGACTGGTTACTGTCACTTG	60	96.7
Mus musculus 18 s	ACACGGACAGGATTGACAGATTG	GCCAGAGTCTCGTTCGTTATCG	60	88
Mus musculus Hprt	AGGGATTTGAATCACGTTTG	TTTACTGGCAACATCAACAG	60	97.3
Mus musculus Ppib	TGGAGATGAATCTGTAGGAC	CAAATCCTTTCTCTCCTGTAG	60	100.4
Mus musculus Vil1	TATGGAGGATCGAGGCTATG	AGGTAGTCATCCATCTGTGT	60	98.9
Mus musculus Lgr5	AGAACACTGACTTTGAATGG	CACTTGGAGATTAGGTAACTG	58	116.2
Mus musculus Cd3e	ATCTTGGTAGAGAGAGCATTC	CCCATTTTAAGTTCTCGTCAC	60	109.4
Rattus norvegicus Tlr2	AAAAACTGCTGAGATTTTGC	TACTAACATCCAACACCTCC	60	84.6
Rattus norvegicus Tlr5	AAAAGCAGCAGTATTTGAGG	ACTAGGAAACCGTCCTTATG	60	86.3
Rattus norvegicus Tlr9	CCTTTGGTTTCCAGTAACATC	CAGAAGTCCATATTCACAGTTC	60	106
Rattus norvegicus Nod2	AACCTCAGCCATAACATCC	ACTTCCAGCAGTAAGTCTAC	60	94
Rattus norvegicus Tlr44	ACCTAGATCTGAGCTTCAAC	TTGTCTCAATTTCACACCTG	60	113.8
Rattus norvegicus 18 s	GGACACGGACAGGATTGAC	TCGCTCCACCAACTAAGAAC	60	96.3