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. 2018 Nov 26;8:17358. doi: 10.1038/s41598-018-35790-w

Figure 1.

Figure 1

BTH compromises Rhizoctonia solani resistance in Brachypodium distachyon accessions Bd3-1 and Gaz-4. Detached leaves of B. distachyon Bd3-1 and Gaz-4 were spray-treated with 0.5% (v/v) DMSO, 0.5 mM SA, or 0.5 mM BTH and 24 h later inoculated with R. solani mycelial plugs (2–3 mm3). Lesion formation (upper panel) and relative biomass of R. solani (lower panel) in (a) Bd3-1 and (b) Gaz-4 leaves were evaluated at 3 d post-inoculation. Data are presented as means ± SEM of values relative to the DMSO treatment, n = 9; **P < 0.01, ***P < 0.001 using Student’s t-tests. The experiments were performed twice with similar results and a representative result is shown.