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. 2018 Nov 26;8:17358. doi: 10.1038/s41598-018-35790-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BTH induces expression of chloroplast- and JA-related genes in Brachypodium distachyon Bd21 and Bd3-1. Detached leaves of B. distachyon were spray-treated with 0.5% (v/v) DMSO, 0.5 mM SA, 0.5 mM JA, or 0.5 mM BTH for 24 h. Expression levels of rbcS genes and BdAOS were evaluated by qRT-PCR analysis in Bd21 (a) and Bd3-1 (b). Data are presented as means ± SEM of values relative to the DMSO treatment, n = 4; *P < 0.05, **P < 0.01, ***P < 0.001 using Student’s t-tests. The experiments were performed twice with similar results and a representative result is shown.