Fig. 4. Cinacalcet upregulates CaSR, CaMKKβ, phospho-Ser⁴²⁸ LKB1, phospho-Thr¹⁷² AMPK, PGC-1α, phospho-Ser¹¹⁷⁷ eNOS, Bcl-1/Bax ratio, Beclin-1, and LC3-II/LC3-I ratio of the sciatic nerve in db/db mice.

a, b The expression levels of CaSR, CaMKKβ, phospho-Ser⁴²⁸ LKB1, phospho-Thr¹⁷² AMPK, PGC-1α, phospho-Ser¹¹⁷⁷ eNOS, Bcl-1, Bax, Beclin-1, LC3-I, and LC3-II of the sciatic nerve were determined at 20 weeks in db/m and db/db mice. Cinacalcet treatment upregulates the expression of these intracellular signal pathway after the 12-week treatment in db/db mice. Representative western blot of CaSR, CaMKKβ, phospho-Ser⁴²⁸ LKB1, phospho-Thr¹⁷² AMPK, PGC-1α, phospho-Ser¹¹⁷⁷ eNOS, and β-actin (a) and quantitative analyses of the results are shown (b). c, d Representative western blot of Bcl-2 and Bax (c) and Beclin-1, LC3-I, and LC3-II (d) and their quantitative analyses of the results are shown (c, d, respectively). (n = 2 in each groups) *p < 0.05, **p < 0.01, and ^#p < 0.001 compared with the other groups