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. 2018 Nov 20;9:1595. doi: 10.3389/fphys.2018.01595

FIGURE 7.

FIGURE 7

Heme induces marked HO1 and ferritin heavy chain expression in a concentration- and time-dependent manner. Cells were treated either with heme or vehicle as mentioned above. HO1 expression was measured using qRT-PCR and immunoblot, while ferritin heavy chain by immunoblot (A–G). (A) Relative expressions of HO1 was determined by qRT-PCR, normalized to GAPDH and compared to the untreated controls at each time points. Results are presented as mean ± SD of five independent experiments performed in duplicates. ∗∗∗p < 0.001. (B,D,F) Representative Western blots of whole cell lysates from five independent experiments are shown representing HO1 and H-ferritin (FTH) protein levels (B) 3, (D) 6, and (E) 16 h after the heme treatment. (C,E,G) Densitometric analysis of FTH expression (C) 3, (E) 6, and (G) 16 h after the heme exposure. Results are presented as mean ± SD of five independent experiments. FTH expressions are normalized to GAPDH and compared to the untreated controls at each time points. UC, untreated control; VC, vehicle control; PC, positive control, thapsigargin treated. ∗∗p < 0.01, ∗∗∗p < 0.001.