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. 2018 Nov 20;9:1595. doi: 10.3389/fphys.2018.01595

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Copyright © 2018 Gáll, Pethő, Nagy, Hendrik, Méhes, Potor, Gram, Åkerström, Smith, Nagy, Balla and Balla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A1M and Hpx attenuates Grp78 and HO1 gene induction. Cells were treated with heme or heme + rA1M or equimolar amonts of heme + Hpx as described in the legend to Figure 8. Grp78 and HO1 expression was measured using qRT-PCR 3 and 16 h after the treatment (A–D). (A) Relative expressions of Grp78 in response to heme and heme + rA1M complex were determined by qRT-PCR, normalized to GAPDH. Grp78 levels of rA1M + heme treated cells were compared to those detected in heme challenged cells. Results are presented as mean ± SD of five independent experiments performed in duplicates. ^∗∗∗p < 0.0001 using unpaired t-test (B) Relative expressions of Grp78 in response to heme and heme + Hpx complex were determined by qRT-PCR, normalized to GAPDH. Grp78 levels of Hpx + heme treted cells were compared to those detected in heme challenged cells. Results are presented as mean ± SD of five independent experiments performed in duplicates. ^∗∗∗p < 0.0001 using unpaired t-test. (C) Relative expressions of HO1 in response to heme and heme + rA1M complex were determined by qRT-PCR, normalized to GAPDH. HO1 levels of rA1M + heme treated cells were compared to those detected in heme challenged cells. Results are presented as mean ± SD of five independent experiments performed in duplicates. ^∗∗∗p < 0.0001 using unpaired t-test. (D) Relative expressions of HO1 in response to heme and heme + Hpx complex were determined by qRT-PCR, normalized to GAPDH. HO1 levels of Hpx + heme treted cells were compared to those detected in heme challenged cells. Results are presented as mean ± SD of five independent experiments. ^∗∗∗p < 0.0001 using unpaired t-test.