Figure 2.

Treatment with DDC decreases oxidative stress, ER stress and apoptosis in the livers of mice fed a MCD diet for 8 weeks. Male C57BL/6 mice were fed either MCD or MCS diets for 8 weeks. The MCD group were treated either with vehicle (1 × PBS) or 450 mg·kg⁻¹ DDC via daily gavage for all 8 weeks. (A) Immunoblot analysis of CYP2E1 in liver lysates of mice. (B) The transcript levels of CHOP in the liver of mice. (C) The transcript levels of ATF4 in the liver of mice. Data are expressed as mean ± SEM; n = 8 mice per group. One‐way ANOVA followed by Dunnett's post hoc test for multiple comparisons. *P < 0.05. (D) Immunohistochemical staining for Bax and cleaved caspase‐3 in liver sections (original magnification 200×).