Figure 4.
Treatment with DDC suppresses hepatic inflammation and macrophage infiltration in the livers of mice fed a MCD diet for 8 weeks. Male C57BL/6 mice were fed either MCD or MCS diets for 8 weeks. The MCD group were treated either with vehicle (1 × PBS) or 450 mg·kg−1 DDC via daily gavage for all 8 weeks. (A) H&E staining of representative liver sections (original magnification 200×) showed hepatic inflammation. (B) Scores of hepatic inflammation in liver sections were measured. (C) The transcript levels of TNF‐α in the liver of mice. (D) The transcript levels of CCR5 in the liver of mice. (E) The transcript levels of CCR2 in the liver of mice. Data are expressed as mean ± SEM; n = 8 mice per group. One‐way ANOVA followed by Dunnett's post hoc test for multiple comparisons. *P < 0.05. (F) Immunohistochemical staining for CD68, CD206 and iNOS of continuous liver sections (original magnification 200×).