Figure 2.

The presence of mast cells accelerates graft rejection in a sex-mismatch MHC ClI-restricted minor transplantation antigen donor ear skin model. (A) Representative images of progressive rejection scores as detailed in Materials & Methods and used for evaluation are shown. (B) Kinetic analysis of rejection in sex-matched and sex-mismatched animals treated with PBS- (+ MC) or DT (- MC) in both host and donor mice as indicated. (C) Kinetic analysis of rejection in sex-mismatched animals treated with PBS- (+ MC) or DT (- MC) in either donor, host or both as indicated. Data in B and C represent means ± SEM of rejection scores from at least 4 independent experiments (total n = 8 to 10 mice/condition. *compares PBS-treated vs. DT-treated (MC-deficient) mice in both host and donor, ^# compares PBS-treated vs. DT-treated (MC-deficient) mice in donor only. Differences between other groups of mice were not statistically significant. Statistic was analyzed using one- way ANOVA test using the nonparametric Bonneferoni multiple comparisons test (comparing all the groups).