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. 2018 Nov 20;9:2690. doi: 10.3389/fimmu.2018.02690

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Copyright © 2018 Ngo Nyekel, Pacreau, Benadda, Msallam, Åbrink, Pejler, Davoust, Benhamou, Charles, Launay, Blank and Gautier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mast cells transiently increase after grafting under both matched and mismatched conditions. Analysis of skin tdT/c-kit positive MC in nongrafted and grafted tissue as indicated. Numbers are absolute numbers of counted MC per ear pair in the indicated conditions following tissue extraction and flow cytometry analysis as detailed under Materials & Methods. Data represent means ± SEM from at least 4 independent experiments (total n = 8 to 10 mice/condition. *compares nongraft vs. graft conditions, ^# compares mast cell sufficient PBS (+ MC) vs. MC-deficient DT (-MC) treated mice, ^§compares matched vs. mismatched conditions using two-way ANOVA test using the nonparametric Bonneferoni multiple comparisons test (comparing all the groups).