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. 2018 Nov 20;9:2690. doi: 10.3389/fimmu.2018.02690

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Copyright © 2018 Ngo Nyekel, Pacreau, Benadda, Msallam, Åbrink, Pejler, Davoust, Benhamou, Charles, Launay, Blank and Gautier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Marked early neutrophil infiltration in ear tissue after grafting is majored under mismatch conditions in the presence of MC. Analysis of neutrophils, monocytes, macrophages, B cells, T cells, and Foxp3-positive regulatory cells in nongrafted and grafted ear tissue at day 2 and 6. Numbers represent absolute numbers of counted cells per ear pair in the indicated conditions following tissue extraction and flow cytometry analysis as detailed under Materials & Methods. Data represent means ± SEM from at least 4 independent experiments (total n = 8 to 10 mice /condition. *compares nongraft vs. graft conditions, ^# compares PBS- (+ MC) vs. DT (–MC) treated mice, ^§compares sex-matched vs. sex-mismatched conditions using two-way ANOVA test using the nonparametric Bonneferoni multiple comparisons test (comparing all the groups).