Figure 5.

Mast cells induce some early transient changes in monocyte and Foxp3-positive cell infiltration in draining lymph nodes after grafting under mismatch conditions. Analysis of neutrophils, monocytes, macrophages, B cells, T cells, and regulatory T cells in draining lymph nodes of nongrafted and grafted draining lymph nodes (cervical in the neck region) at day 2 and 6. Numbers represent absolute numbers of counted cells in pooled lymph nodes in the indicated conditions following tissue extraction and flow cytometry analysis as detailed under Materials & Methods. Data represent means ± SEM from at least 4 independent experiments (total n = 8 to10 mice/ group. *compares nongraft vs. graft conditions, ^# compares PBS- (+ MC) vs. DT (–MC) treated mice, ^§compares sex-matched vs. sex-mismatched conditions using two-way ANOVA using the nonparametric Bonneferoni multiple comparisons test (comparing all the groups).