Figure 7.

Deletion of neutrophils delays rejection. (A) C57BL/6N mice were treated twice at 16 h before grafting and at Day 4 after grafting i.p. with 200 μg of anti-neutrophil (NIMP-R14) or isotype control antibodies. Representative images of the assessment of neutrophil depletion shows dot blots of the neutrophil population in control and anti-neutrophil treated animals at indicated times. (B) Kinetic analysis of rejection in sex-mismatched animals treated with control and anti-neutrophil antibody in the host as indicated. Data represent means ± SEM of rejection scores from at least 4 independent experiments (total n = 8 to 10 mice/condition) using one-way ANOVA using the nonparametric Bonneferoni multiple comparisons test (comparing all the groups).