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. 2018 Nov 20;9:2690. doi: 10.3389/fimmu.2018.02690

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Copyright © 2018 Ngo Nyekel, Pacreau, Benadda, Msallam, Åbrink, Pejler, Davoust, Benhamou, Charles, Launay, Blank and Gautier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MCs degranulate after grafting both under matched and sex-mismatch conditions (A) Representative photomicrographs of ear skin from nongrafted and sex-mismatched grafted tissue stained with toluidine blue. Degranulated MC visible in allogenic grafts are indicated by arrows. The inset show a high magnification picture of a nondegranulated and a degranulated MC (B) Quantitative evaluation of degranulated MC in nongrafted as well as matched and sex-mismatched grafted tissues. Scale bar: 100 μm; insert scale bar: 10 μm. Data represent the percentage of degranulated MC (means ± SEM) present 8 high power fields counted from at least three independent experiments using the Student's t test. No significant differences were observed between matched and mismatched conditions.