FIG 2.

Pyruvate kinase activity in B. abortus 2308 and ΔmntH and ΔpykM mutant cells grown to mid-log phase in a Mn-restricted defined medium. The data represent the results of 3 assays performed at different times. The baseline levels of NADH to NAD conversion in reaction mixtures not containing cell lysate or PEP have been subtracted from the values presented. *, P  < 0.05 for comparisons of 2308 versus the mntH and pykM mutants by least significant difference one-way analysis of variance (LSD-ANOVA).