TABLE 2.
Oligonucleotide primers used in this study
| Primer | Sequence (5′→3′) |
|---|---|
| PykM up F BamHI | GCAGGATCCTCATCAATAAGACTGCCCCG |
| PykM up R | GGCATTTAGAGCGCATCCCG |
| PykM down F | TGCGCTTCATTGATTATTCG |
| PykM down R PstI | GCACTGCAGACAATTGCATGATGAGGGTC |
| pykM conf F | ATCGATGGTTCAAGCTGCAA |
| pykM conf R | CGCCATGACTGGATGATATT |
| mntH up F | ATCGTCGACCGGACGTTCCGGAATTTCATCGACACT |
| mntH up R | TACCATATGAAATACCTTTATCCTTTATGCAG |
| mntH down F | ATTTAAAGCACGTTTCCCGAAAGGGCG |
| mntH down R | ACGGGGCCCTCTCTCCCAAGGGCGGTCCAATAAAAGAA |
| mntH conf F | GGCGGATTCGTTCTTCAGTT |
| mntH conf R | GAAAGGGTGGGGTAAGAGCC |
| ppdK upF SpeI | GATACTAGTCGCACATGGGTTTTGCTCAAGG |
| ppdK upR | TGCCATTCCGGCCTCCCTTGACACAGGACAAGGTTCCCGAAAG |
| ppdK downF | GTGTAAACCGCTGCCATAATTCA |
| ppdK downR PstI | GATCTGCAGTCTTAGTCGCATTATCCTAC |
| ppdK confF | CTCCGATAAGTTCCCCATTT |
| ppdK confR | CGCTGAAAACTTTTGAAACATG |
| edd upF PstI | GATCTGCAGTTTGAACGATTGTCATACGG |
| edd upR | TTCTGAAACAGCGCGAATAAAATGA |
| edd downF | GGACATGGGTATCACCTGTGCC |
| edd downR EcoR1 | GATGAATTCATGAGCGCCTCATCATGGATGTGGTGC |
| edd confF | ATGTGGGTATCAACCGCATC |
| edd confR | CTTCCTTCTTCCCCAATGCC |
| rpykM BamHI F | GGATCCAAGCGCAACCGCAAGGTCAA |
| rpykM HindIII R | AAGCTTCTAAATGCCGGATTTTCCGT |