FIG 4.

Gel shift assay using LitR recombinant protein. LitR (0 to 80 fmol) was incubated with the probes for PlitR (306 bp), P5689 (378 bp), and PrpoD (436 bp) and loaded into a nondenaturing polyacrylamide gel. LitR was illuminated with UV-A at 0.06 μmol · s⁻¹ · m⁻² for 180 s prior to the addition of Cy5-labeled probe. Open and closed arrowheads indicate the probe alone and protein-probe complex, respectively.