FIG 6.

Gel filtration chromatography for the analysis of light-induced dissociation of LitR protein. To estimate the relative molecular masses of the nonilluminated (solid line) and UV-A-illuminated (dashed line) LitR, the recombinant protein LitR was loaded onto a Superdex 200 10/300 GL column and the absorbance spectra were monitored at 280 and 340 nm. The molecular mass standards used were ferritin (Fe), conalbumin (Al), carbonic anhydrase (Ca), and RNase (Rn), indicating 440, 75, 29, and 13.7 kDa, respectively. AU, arbitrary units.