FIG 2.

Cre is expressed in Chlamydia spp. and does not impact development. HeLa cells were infected for 24 h with equivalent IFUs of C. trachomatis expressing (+CRE) or lacking (-CRE) pSU-CRE. (A) Whole-culture material was probed in immunoblots with Cre-specific antibodies or anti-Hsp60 as the loading control and visualized via chemiluminescence. (B) Cultures were methanol fixed and stained for inclusion visualization using indirect immunofluorescence. Areas of 50 representative inclusions were measured and plotted individually, with the means and standard deviations shown. (C) Primary cultures were harvested, and progeny Chlamydia spp. were enumerated after secondary passage onto fresh HeLa cells.