FIG 6.

L2^Rif tmeA-lx manifests a developmental defect in tissue culture. (A) HeLa cultures were infected in triplicate with equal numbers of Chlamydia spp. (A) or IFUs (B) using L2^Rif (WT) + pCompAII or L2^Rif tmeA-lx expressing pCompAII (vector) or ptmeA to achieve an approximate MOI of 0.1. Cultures were methanol fixed and stained for chlamydiae (left) or processed for enumeration of progeny EBs (right) at 24 hpi. All inclusions were enumerated by fluorescence staining of chlamydiae in fixed samples, and data for direct and progeny counts are represented as mean ± standard deviation of triplicate samples. A Student t test with Welch’s correction was used to address significance (*, P < 0.02; **, P < 0.005).