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. 2018 Nov 20;9:1686. doi: 10.3389/fpls.2018.01686

FIGURE 2.

FIGURE 2

Analysis of mll2707 and mlr0970 gene expression in M. loti MAFF303099 wild-type and tatC mutant strains. The expression level of mll2707 and mlr0970 genes was determined by real time RT-PCR assays. Below each bar the strain and the gene assayed are indicated. Data correspond to triplicates in at least three independent experiments. mRNA was extracted from wild-type (wt) and tatC mutant strains grown in solid media under aerobiosis (O2) (A), microaerophilic conditions (Micro) (B), and anaerobiosis (Anaerob) (C). D,E Comparative relative expression level of mll2707 (D), and mlr0970 (E) genes in the wild-type strain growing under the different oxygen conditions in vitro and in nodules (Nod). Errors bars correspond to standard deviations from replicates. Asterisks indicate a p-value <0.05 (∗) or 0.01 (∗∗) in Student’s t-tests.