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. 2018 Nov 20;9:1686. doi: 10.3389/fpls.2018.01686

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Copyright © 2018 Basile, Zalguizuri, Briones and Lepek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytochrome oxidase activity in M. loti MAFF303099 wild-type, mutant, and complemented mutant strains. Disks impregnated with N,N-dimethyl-p-phenylenediamine (oxalate) were added to 60 μl of wild-type (wt), tatC, mll2707, mlr0970, and mll2707/mlr0970 mutant strains, tatC mutant strain complemented with tatC gene cloned in pBBR1MCS-2 (tatC strain plus tatC), and mll2707 mutant strain complemented with mll2707 gene in pBBR1MCS-4 (mll2707 strain plus mll2707) (in this case the mll2707-3XFLAG construction was used for complementation). Development of a pink coloration was evaluated within 1 min.