FIGURE 2.

EC₅₀ of T315 against S. Typhimurium biofilms is 7.4 μM. (A) Determination of the half-maximal effective concentration of T315 was achieved by exposing S. Typhimurium to drug concentrations from 1 to 100 μM and examining the levels of subsequent anti-biofilm activity after 24 h. (B) EC₅₀ [7.4 μM (95% CI, 6.3–8.6 μM)] calculated using GraphPad Prism 7 to plot normalized T315 activity (percent of biofilm inhibition) as a function of log₁₀ drug concentration and fitting of the dose-response curve (log[agonist] vs. normalized response, variable slope).