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. 2018 Nov 20;9:2804. doi: 10.3389/fmicb.2018.02804

FIGURE 5.

FIGURE 5

T315 targets WrbA to inhibit S. Typhimurium biofilm formation. (A) In the absence of drug treatments, S. Typhimurium ΔwrbA establishes reduced biofilm formation, 22.4% on average, relative to wildtype (∗∗∗p < 0.001; unpaired t-test). The addition of 20 μM T315 reduced biofilm formation in both S. Typhimurium wildtype and ΔwrbA strains (∗∗p < 0.01, ∗∗∗p < 0.001; ratio paired t-test), though its anti-biofilm activity is weakened by loss of WrbA. (B) Transformed data clearly show that T315 anti-biofilm activity with 20 μM treatment was significantly reduced in ΔwrbA compared to wildtype, by an average of 30.3%, indicating a reliance on WrbA to achieve maximum biofilm inhibition (∗∗∗∗p < 0.0001; unpaired t-test).