Table 3.
Signalling pathway transduction ratios (logR) and transducer function slopes (“n”), with between-pathway delta–delta transduction ratios (ΔΔlogR) and bias factors relative to reference ligand CP 55,940.
| logR [log(τ /KA)] (±SEM) |
cAMP – pERK |
cAMP – internalisation |
Internalisation – pERK |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Compound # | Internalisation | cAMP | pERK | ΔΔlogR (±SEM) | Bias factor | ΔΔlogR (±SEM) | Bias factor | ΔΔlogR (±SEM) | Bias factor |
| CP 55,940 | 8.76 (0.1) | 8.52 (0.02) | 8.05 (0.03) | 0.00 (0.04) | 1.0 | 0.01 (0.10) | 1.0 | –0.02 (0.11) | 1.0 |
| 7 | 7.42 (0.26) | 7.29 (0.26) | 5.51 (0.05) | 1.31 (0.27)*** | 20.3 | 0.12 (0.31) | 1.3 | 1.19 (0.16)*** | 15.4 |
| 10 | 7.09 (0.10) | 6.90 (0.10) | 6.06 (0.02) | 0.37 (0.10) | 2.4 | 0.07 (0.16) | 1.2 | 0.31 (0.13) | 2.0 |
| 14 | 6.81 (0.23) | 5.83 (0.23) | 5.16 (0.09) | 0.20 (0.25) | 1.6 | –0.73 (0.25)* | 0.2 | 0.93 (0.13)** | 8.6 |
| 15 | 8.92 (0.09) | 8.12 (0.09) | 7.45 (0.10) | 0.20 (0.13) | 1.6 | –0.56 (0.15) | 0.3 | 0.76 (0.15)* | 5.7 |
| 16 | 8.90 (0.19) | 8.52 (0.19) | 7.84 (0.07) | 0.21 (0.20) | 1.6 | –0.14 (0.22) | 0.7 | 0.35 (0.13) | 2.2 |
| nΔ | 1.49 (0.21) | 1.28 (0.14) | 1.14 (0.06) | ||||||
The symbol “∗” indicates significant difference from CP 55,940 for the same bias comparison; significance levels as defined in the methods. Δ“n” is the transducer function slope which was determined for each signalling pathway as a shared constraint between ligands when fitting the operational model.