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. 2018 Nov 20;8:538. doi: 10.3389/fonc.2018.00538

Figure 5.

Figure 5

Knock-down of β-catenin attenuates the effect of SIRT overexpression in invasion activity of ovarian cancer cells. (A) The MTT assay was performed after overexpression of SIRT6 with or without knock-down of β-catenin. (B,C) SIRT6 was overexpressed via a plasmid containing wild-type SIRT6 and β-catenin was knocked-down with shRNA for β-catenin in OVCAR5 ovarian cancer cells. Wound healing assays (B), and migration and invasion assays (C) were performed after overexpression of SIRT6 with or without knock-down of β-catenin in OVCAR5 cells. The number of cells which migrated or invaded the chambers were counted in five microscopic fields per well at one-hundred magnification. (D) Western blotting was performed for SIRT6, β-catenin, active β-catenin, snail, vimentin, N-cadherin, E-cadherin, and actin after overexpression of SIRT6 with or without knock-down of β-catenin in OVCAR5 cells. (E) The mRNA expression patterns for SIRT6, β-catenin, snail, vimentin, N-cadherin, and E-cadherin were analyzed via quantitative reverse-transcription polymerase chain reaction after overexpression of SIRT6 with or without knock-down of β-catenin in OVCAR5 cells. CV, control vector; OE, overexpression; the P values were calculated by one-way ANOVA with LSD test; *, vs. control; P < 0.05, **, vs. control; P < 0.001, #, vs. SIRT6 overexpression; P < 0.05.