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. 2018 Nov 20;9:1635. doi: 10.3389/fphys.2018.01635

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Copyright © Bogdanova, Kostina, Zihlavnikova Enayati, Zabirnyk, Malashicheva, Stensløkken, Sullivan, Kaljusto, Kvitting, Kostareva, Vaage and Rutkovskiy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Partition of the groups based on the results of principal component analysis of gene expression in VICs cultured on collagen or elastin and stimulated by LPS, stretch or both. Principal component analysis was used to investigate does treatment with LPS or/and stretch divide VICs cultured on collagen or elastin by different groups. Expression data on a set of the following genes was used: ICAM1, BMP2, RUNX2, POSTN, THBS1, ACTA2, and CNN1. Scatter plot demonstrates that cells from calcified valves cultured on collagen (in green) and elastin (in red) form separate clusters by gene expression after simulation by LPS (Flex-LPS+) or stretch (Flex+LPS-) alone, but not in combination (Flex+LPS+). Cells from healthy valves do not form separate clusters in all cases. Each dot on scatter plot represents individual cell line (cell replicate in experiments).