Chart 1.

Genes included in TruSeq Targeted RNA Custom Panel Kit and the functions and routes involved in the development of vitiligo

Pathway	Gene	Function
Pigmentation	DCT8,30	Involved in regulating eumelanin and pheomelanin levels
	MC1R 8	Encodes the receptor protein for melanocyte-stimulating hormone (MSH); controls melanogenesis
	MC4R 8	Encoded a protein that interacts with adrenocorticotropic and MSH hormones and is mediated by G proteins
	POMC8	Encodes a preproprotein that undergoes extensive, tissue-specific, post-translational processing via cleavage. One peptide produced from the POMC protein is a-MSH involved in regulating the pig­ment-producing cells of the skin and hair (melanocytes), where it binds to melanocortin 1 receptor (MC1R)
	TYRP18,30	Encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway
	MLANA30	Involved in melanosome biogenesis
	PHACTR227	Phosphatase and actin regulator 2
Apoptosis	BAX25	BCL2-associated X protein that acts as an anti- or pro-apoptotic regulator that is involved in a wide variety of cellular activities
	BCL29,25	Encodes an integral outer mitochondrial membrane protein that blocks apoptotic death
	BCL326	Involved in the regulation of cell proliferation and contributes to transcriptional regulation of NFKB
	CASP325,29	Is a member of the cysteine-aspartic acid protease (caspase) family; plays a central role in the execu- tion-phase of cell apoptosis
	CASP731	Is a member of the cysteine-aspartic acid protease (caspase) family; plays a central role in the execu- tion-phase of cell apoptosis
	CASP829	Is a member of the cysteine-aspartic acid protease (caspase) family; plays a central role in the execu- tion-phase of cell apoptosis.
	CASP1032	Is a member of the cysteine-aspartic acid protease (caspase) family; plays a central role in the execu- tion-phase of cell apoptosis
	CFLAR28	CASP8 and FADD-like apoptosis regulator, is a regulator of apoptosis and is structurally similar to caspase-8
	FASLG35	Fas ligand (TNF superfamily, member 6); induction of apoptosis triggered by binding to FAS
	TNF37	Tumor necrosis factor, involved in the regulation of a wide spectra of biological processes, including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation
	TNFRSF1A33	One of the major receptors for the tumor necrosis factor-alpha; activates NF-kappa B, mediates apoptosis, and functions as a regulator of inflammation
Oxidative stress	GGT134	Involved in the maintenance of intracellular GSH levels. It is part of the cellular antioxidant defense mechanism
	CCBL242	Cysteine conjugate-beta lyase 2, involved in the regulation of oxidative stress
	GPD138	Glycerol-3-phosphate dehydrogenase 1, involved in the redox metabolism
	TXN39	Thioredoxin, inhibits oxidative stress and caspase 3 activity
Cell survival	CAPN310	A major intracellular protease involved in melanocyte survival
	MITF9,30	Involved in melanocyte survival, controls the expression of genes that relate melanin synthesis
	CDC5L36	Involved in cell cycle control
	MAPK140	Mitogen-activated protein kinase 1, involved in cell proliferation and differentiation
Signal	CSNK1G340	Participates in the Wnt signaling pathway
transduction	NFKB126	Nuclear Factor Kappa B Subunit 1, is a transcription regulator that stimulates the expression of genes involved in a wide variety of biological functions
	WNT7A41	Wnt Family Member 7A, implicated in oncogenesis and several developmental processes
Endogenous control / constitutive expression	TPT1	Tumor protein, translationally controlled 1, involved in calcium binding and microtubule stabiliza­tion. Endogenous control / constitutive expression
	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase. Endogenous control / constitutive expression