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. 2018 Nov 20;12:284. doi: 10.3389/fnbeh.2018.00284

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Copyright © 2018 Phi Van, Krause and Phi-Van.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Serotonin transporter (5-HTT) genotyping using restriction endonucleases. PCR was performed with 0.5 μg genomic DNA from W/W, W/D or D/D chicks and DNA primers 281 and 282. PCR products were digested without (lanes 1, 4 and 7) or with restriction endonucleases Vsp I (lanes 2, 5 and 8) or Mun I (lanes 3, 6 and 9) or with both enzymes (lane 10). Digested DNA fragments were then resolved by electrophoresis on a 2% agarose gel, stained with ethidium bromide and visualized under UV-illumination. Asterisk (*) indicates the ~0.4-kb heteroduplex formed from W and D. DNA markers used were the 100-bp ladder (lane M).